P. berghei is one of the four malaria species that infect murine rodents from Central Africa that are infectious to laboratory rodents and easily maintained and transmitted by Anopheles stephensi in the laboratory. It serves as a good model for the human parasites with which it shares high homology in most essential aspects of structure, biochemistry and life cycle. Genome organisation is conserved between rodent and human parasites and the two share detailed synteny.
P. berghei has an estimated genome size of 18-20 Mb, with 14 chromosomes in the size range of 0.6 Mb to 3.8 Mb. DNA for this sequencing project was obtained from asynchronous bloodstages of clone 15cy1 of the ANKA strain of P. berghei.
The genome of P. berghei ANKA has been published A comprehensive evaluation of rodent malaria parasite genomes and gene expression.
» Plasmodium berghei on GeneDB
An improved genome for Plasmodium berghei ANKA (version 3) is now available
We improved the genome of P. berghei ANKA using long reads technologies (PacBio) (July 2015). We were able to close all the sequencing gaps in the core regions of the genome, resolve false duplications and so decreasing the amount of unassigned contigs to 5. Due to the nature of the repetitive structure (2.3kb repeats in tandem), we are still not able to produce 14 fully assembled chromosomes with the current sequencing technologies. The annotation has been manually re-inspected genome-wide. Due to the rearrangement in the sequence, we were forced to rename the gene id, mostly adding a zero. A lookup table between the genes of version 2 and the new version 3 can be found on the FTP site. It also includes duplicated and novel genes.
A publication describing the new assembly is in preparation and permission should therefore be obtained before undertaking genome-scale or chromosome wide analysis. Please refer to  Data Use Statement.
This work was funded by the Wellcome Trust Sanger Institute. The DNA was provided by Oliver Billker’s Lab (Wellcome Trust Sanger Institute). The assembly was performed by Thomas D. Otto. For further information please contact firstname.lastname@example.org.