Protozoan parasites within the species Trypanosoma brucei are the etiological agent of human sleeping sickness and Nagana in animals. Infections are limited to patches of sub-Saharan Africa where insects vectors of the Glossina genus are endemic. The most recent estimates indicate between 50,000 - 70,000 human cases currently exist, with 17 000 new cases each year (WHO Factsheet, 2006).
For more information/stats, etc., we recommend the WHO website.
T. brucei possesses a two-unit genome, a nuclear genome and a mitochondrial (kinetoplast) genome with a total estimated size of 35Mb/haploid genome. The nuclear genome is split into three classes of chromosomes according to their size on pulsed-field gel electrophoresis, 11 pairs of megabase chromosomes (0.9-5.7 Mb), intermediate (300-900 kb) and minichromosomes (50-100 kb). The T. brucei genome contains a ~0.5Mb segmental duplication affecting chromosomes 4 and 8, which is responsible for some 75 gene duplicates unique to this species. A comparative chromosome map of the duplicons can be accessed here (PubmedID 18036214).
GeneDB has been funded by Wellcome Trust to provide full curation support for kinetoplastida genomes to meet the needs of the community. New annotations are constantly being added to keep up with published manuscripts and feedback from the Trypanosomatid research community. In collaboration with GeneDB, the EuPathDB genomic sequence data and annotations are regularly deposited on TriTrypDB where they can be integrated with other datasets and queried using customized queries.
The genome of the T. brucei TREU 927 has been published in Berriman et al. 2005, Science 309(5733):416-422 and is available for use without restriction. However, the annotation is pro-actively updated and curated. If you find the this resource helpful in your research, acknowledging the project in your publications will help insure its longevity.
T. brucei 927 functional annotation has been updated in TriTrypDB 9.0 (products, GO annotation, EC numbers, gene names and synonyms; GeneDB v20141120).
Summary of annotation changes since previous release (GeneDB v20130801): Annotation updates
Note: Only gene names, products, GO annotation and EC numbers have been updated in TriTrypDB 9.0. Genome sequence for T. brucei 927 is still v5.1 (the same currently available at GeneDB). Gene models and other functional annotation correspond to GeneDB v20130801.
Download the latest annotation (gff3 files generated each month):
FTP - Rolling releases: current GFF3 'Tbruceibrucei927' files.
Note: Genome sequence for T. brucei 927 is still v5.1 (the same currently available at TriTrypDB). Genome sequence (fasta files) can also be downloaded from the ftp link above.
T. brucei 927 version 5.2 (November 2013) - Version currently on TriTrypDB
New sequence and annotation files are available for T. brucei TREU 927. Please see the readme_v5.2 for a summary of the changes:
FTP - version 5.2_20131108
Note: This update only includes changes in annotation. Genome sequence for T. brucei 927 main chromosomes is still v5.1 (the same currently available at TriTrypDB).
T. brucei 927 version 5.1 (August 2013)
New sequence and annotation files are available for T. brucei TREU 927. Please see the readme_v5.1 for a summary of the changes:
FTP - version 5.1_20130801
Note: This update contains changes in annotation and a new sequence and annotation for chromosome 11 bin (unassembled contigs from chromosome 11). Genome sequence for T. brucei 927 main chromosomes is still v5.
New versions of Chromosomes 9 and 11 (Dec 2011)
New versions of Chromosome 9 (December 2011) and Chromosome 11 (November 2011) of Trypanosoma brucei TREU 927 are now available. Systematic IDs have been rationalized across these chromosomes to bring the naming structure in line with chromosomes 1-8 and 10. The previous systematic IDs have been stored and remain searchable, and files with all previous and new systematic IDs for these chromosomes are available by following the links below.
Chromosome 9 - Previous Systematic IDs
Chromosome 11 - Previous Systematic IDs
Comparative analysis of surface-expressed gene families