Controlled Curation: 
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Category - ControlledCuration Count
expressed in procyclics and bloodstream form 30
expressed in promastigotes 24
expressed in promastigotes and amastigotes 4
expressed in stumpy form 2
expressed in trypomastigotes 1
Expression levels similar in the procyclic and the bloodstream form 7
expression profile: mRNA upregulated in procyclic form 30
forms high-affinity heterodimer with AdoMetDC 1
gene deletion phenotype: abnormal cell proliferation in bloodstream trypomastigote stage 1
gene deletion phenotype: cross resistance to nitroheterocyclic drugs 2
gene deletion phenotype: decreased cell proliferation in epimastigote stage 2
gene deletion phenotype: essential for cell proliferation 1
gene deletion phenotype: essential in bloodstream trypomastigote stage 3
gene deletion phenotype: increased cell proliferation in procyclic trypomastigote stage 1
gene deletion phenotype: increased proline metabolism 1
gene deletion phenotype: normal cell proliferation in procyclic form 1
gene deletion phenotype: renders parasites uninfective to Vero cells 2
gene order in locus different compared to other strains 10
HDP2, variable repetitive sequence 1
Immunoblotting:protein:expressed in:free:merozoites 1
Immunoblotting:protein:expressed in:late-stage:schizonts(cultured) 1
interacts with ATG12-ATG5 conjugate 1
intergenic spacer, IGS 1
in vitro culture assay with 2,4-diaminoquinazoline: lethal during bloodstream stage 1
in vitro culture assay with 2-hydroxybenzaldehyde-5-phosphate: lethal during bloodstream stage 1
in vitro culture assay with 2-iminobezimidazole: lethal during bloodstream stage 1
in vitro culture assay with 5'-deoxy-5'-(isodomethlene)adenosine and 6-N-Cyclopropyladenosin analogues: lethal during bloodstream stage 1
in vitro culture assay with 6-diazo-5-oxo-1-norleucine: lethal during bloodstream stage 1
in vitro culture assay with acivicin (glutamine analogue): lethal during bloodstream stage 1
in vitro culture assay with acyl hydrazoide: lethal during bloodstream stage 1