|
| Now in the final stages of finishing, the 908,516bp sequence represents an ordered set of contigs.
A small number of gaps remain, which are represented by strings of 100 Ns for physical gaps and
of 50 Ns for sequence gaps in the primary sequence. The ordered contig sequence was produced by
Dr. Kiew-Lian Wan's group at
the Malaysia Genome Institute. A HAPPY map constructed
by Dr. Paul Dear's group at the LMB, Cambridge, UK
was used as a tool to anchor and order the contigs.
The sequence has been manually annotated by Dr. Pierre Rivailler from
the Institute for Animal Health using automatically
generated results from an analysis pipeline developed at the PSU by Arnaud Kerhornou and Marie-Adèle Rajandream. |
|
| An ~8x coverage whole genome shotgun of the Houghton strain of E. tenella was obtained as
part of a collaboration, funded by the BBSRC, between The Wellcome Trust Sanger Institute and the
Institute for Animal Health. The shotgun reads, together
with ~10,000 scaffolding BAC ends, have been assembled into contigs. These data are preliminary, and
as such may be incomplete and/or contain errors. Annotation, including gene predictions, have been
automatically generated, and thus may also contain errors. |
|
All gene predictions for the whole genome contig set were done by Aaron J. Mackey from
the Penn Center for Bioinformatics who trained the
prediction tool Twinscan for Eimeria tenella
using T. gondii as the reference genome. For chromosome 1 a number of gene prediction
tools were used to guide the manual annotation including Twinscan.
The GlimmerHMM predictions for chromosome 1 were generated by Xikun Wu at the Institute for Animal Health. SNAP and genefinder (C. Wilson, L. Hillier & P. Green, unpublished) predictions were generated at the PSU. |
|
| The sequence similarity results include matches to cDNAs from the following sources: those entries
deposited in the EMBL database by
The WashU-Merck Eimeria tenella project,
those reported in "A survey of genes in Eimeria tenella merozoites by EST sequencing"
(International Journal for Parasitology Volume 29, Issue 12 , December 1999, Pages 1885-1892,
also deposited in the EMBL database) and those made available to us by Drs. A. Gruber and Alda Madeira of
The Eimeria ORESTES Project. |
